Microfluidic 3D printing polyhydroxyalkanoates-based bionic skin for wound healing

Wentai Guo; Xiaocheng Wang; Chaoyu Yang; Rongkang Huang; Hui Wang; Yuanjin Zhao

doi:10.1088/2752-5724/ac446b

Volume 1 Issue 1

March 2021

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Wentai Guo, Xiaocheng Wang, Chaoyu Yang, Rongkang Huang, Hui Wang, Yuanjin Zhao. Microfluidic 3D printing polyhydroxyalkanoates-based bionic skin for wound healing[J]. Materials Futures, 2022, 1(1): 015401. doi: 10.1088/2752-5724/ac446b

Citation:

Wentai Guo, Xiaocheng Wang, Chaoyu Yang, Rongkang Huang, Hui Wang, Yuanjin Zhao. Microfluidic 3D printing polyhydroxyalkanoates-based bionic skin for wound healing[J]. Materials Futures, 2022, 1(1): 015401. doi: 10.1088/2752-5724/ac446b

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Microfluidic 3D printing polyhydroxyalkanoates-based bionic skin for wound healing

Wentai Guo^1,2,3
Xiaocheng Wang^3,5
Chaoyu Yang^3,5
Rongkang Huang^1,2,3, ,
Hui Wang^1,2, ,
Yuanjin Zhao^3,4,5, ,

Materials Futures, Volume 1, Number 1

Received Date: 2021-10-25
Accepted Date: 2021-12-16
Publish Date: 2022-01-18

Article information

doi: 10.1088/2752-5724/ac446b

1 Department of Colorectal Surgery, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, People's Republic of China
2 Institute of Biomedical Innovation and Laboratory of Regenerative Medicine and Biomaterials, Biomedical Material Conversion and Evaluation Engineering Technology Research Center of Guangdong Province, Guangzhou 510655, People's Republic of China
3 Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, People's Republic of China
4 State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People's Republic of China
5 Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou 325001, People's Republic of China

Funds:

WTG and XCW contributed equally to this paper. This work was supported by the National Key Research and Development Program of China (Grant No. 2020YFA0908200), the National Natural Science Foundation of China (Grant Nos.52073060 and 61927805), and the Sun Yat-sen University Clinical Research 5010 Program (Grant No. 2017008).

Abstract

Abstract

Biomimetic scaffolds with extracellular matrix (ECM)-mimicking structure have been widely investigated in wound healing applications, while insufficient mechanical strength and limited biological activity remain major challenges. Here, we present a microfluidic 3D printing biomimetic polyhydroxyalkanoates-based scaffold with excellent mechanical properties and hierarchical porous structures for enhanced wound healing. This scaffold is composed of poly(3-hydroxybutyrate-4-hydroxybutyrate) and polycaprolactone, endowing it with excellent tensile strength (2.99 MPa) and degradability (80% of weight loss within 7 d). The ECM-mimicking hierarchical porous structure allows bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) to proliferate and adhere on the scaffolds. Besides, anisotropic composite scaffolds loaded with BMSCs and HUVECs can significantly promote re-epithelization, collagen deposition and capillary formation in rat wound defects, indicating their satisfactory in vivo tissue regenerative activity. These results indicate the feasibility of polyhydroxyalkanoates-based biomimetic scaffolds for skin repair and regeneration, which also provide a promising therapeutic strategy in diverse tissue engineering applications.
- microfluidics,
- 3D printing,
- tissue engineering,
- biomaterial,
- P34HB,
- wound healing

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References(44)

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