rADSC-loaded tubular units composed of multilayer electrospun membranes promoted bone regeneration of critical-sized skull defects

Huamin Jiang; Zhaoyi Lin; Jinze Li; Ting Song; Hongyun Zang; Pengwen Li; Jiarun Li; Wenyi Hou; Jianhua Zhou; Yan Li

doi:10.1088/2752-5724/ad66ea

September 2024

Article Contents

Huamin Jiang, Zhaoyi Lin, Jinze Li, Ting Song, Hongyun Zang, Pengwen Li, Jiarun Li, Wenyi Hou, Jianhua Zhou, Yan Li. rADSC-loaded tubular units composed of multilayer electrospun membranes promoted bone regeneration of critical-sized skull defects[J]. Materials Futures, 2024, 3(3): 035403. doi: 10.1088/2752-5724/ad66ea

Citation:

Huamin Jiang, Zhaoyi Lin, Jinze Li, Ting Song, Hongyun Zang, Pengwen Li, Jiarun Li, Wenyi Hou, Jianhua Zhou, Yan Li. rADSC-loaded tubular units composed of multilayer electrospun membranes promoted bone regeneration of critical-sized skull defects[J]. Materials Futures, 2024, 3(3): 035403. doi: 10.1088/2752-5724/ad66ea

Citation:

Huamin Jiang, Zhaoyi Lin, Jinze Li, Ting Song, Hongyun Zang, Pengwen Li, Jiarun Li, Wenyi Hou, Jianhua Zhou, Yan Li. rADSC-loaded tubular units composed of multilayer electrospun membranes promoted bone regeneration of critical-sized skull defects[J]. Materials Futures, 2024, 3(3): 035403. doi: 10.1088/2752-5724/ad66ea

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rADSC-loaded tubular units composed of multilayer electrospun membranes promoted bone regeneration of critical-sized skull defects

Huamin Jiang^{1, 2}
Zhaoyi Lin^{1, 2}
Jinze Li^{1, 2}
Ting Song^{1, 2}
Hongyun Zang^{1, 2}
Pengwen Li^{1, 2}
Jiarun Li³
Wenyi Hou⁴
Jianhua Zhou^{1, 2}
Yan Li^{1, 2, ,}

Materials Futures, Volume 3, Number 3

Received Date: 2024-04-21
Accepted Date: 2024-07-21
Rev Recd Date: 2024-07-09

Article information

doi: 10.1088/2752-5724/ad66ea

1.
School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, People’s Republic of China
2.
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, Sun Yat-sen University, Guangzhou 510006, People’s Republic of China
3.
Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Institute of Stomatological Research, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510062, People’s Republic of China
4.
The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, People’s Republic of China
Declaration of Interests

Yan Li and Sun Yat-sen University have patent protection including China patent number 201 911 230 012.2 ‘fabrication and application of bilayer composite nanofiber membrane with three-dimensional deformation structure’.

Both authors contributed equally.

Abstract

Abstract

AbstractAdipose-derived stem cells (ADSCs) have considerable potential for bone regeneration. However, their performance is limited by a lack of scaffolds that adequately mimic the hierarchical structure of bone to promote proliferation and osteogenic differentiation of ADSCs. In this study, nanofiber membranes composed of polycaprolactone, poly(lactide-co-glycolide), and hydroxyapatite (HAp) were prepared via electrospinning, and the membranes curled after responding to temperature stimuli in an aqueous solution. Transmission electron microscopy and scanning electron microscopy observations indicated that needle-like HAp nanoparticles with an average diameter of 57 ± 39 nm and a length-diameter ratio of 7.4 ± 1.56 were entrapped in the nanofiber matrix and did not affect the surface morphology of fibers. After cutting and deformation, the nanofibers changed from straight to bent, and the diameters increased; they were 1105 ± 200 nm for BPLG85-H and 1120 ± 199 nm for BPLG80-H. Additionally, tubular units with a single layer (BPLG-H(1.5)) or multiple layers (BPLG-H(3.5)) were obtained by controlling the initial shape and size of the membranes. rADSCs on the concave surface of BPLG-H(3.5) proliferated faster and exhibited better osteogenic activity than those on the convex side of BPLG-H(3.5) and both surfaces of BPLG-H(1.5), which was correlated with the higher expressions of vascular endothelial growth factor and bone morphogenetic protein 2. Additionally, rADSCs on both units maintained osteogenic activity after storage at -80 °C for 20 d. In rat skull defect (diameter of 8 mm) models, rADSC-loaded BPLG-H(3.5) units fixed using gelatin hydrogel (ADSC@BHM) exhibited 84.1 ± 6.6% BV/TV after implantation for 12 weeks, which was 155.6% higher than that of the Blank group. H&E and Masson’s staining results demonstrated that there was more bone regeneration at the defect center of ADSC@BHM than in the BHM and Blank groups. In conclusion, rADSC-loaded BPLG-H(3.5) with an osteon-mimic structure provides a potential strategy to repair bone defects.
- adipose-derived stem cells,
- electrospinning,
- composite fiber,
- bone regeneration

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Author contributions

Huamin Jiang, Zhaoyi Lin, Jianhua Zhou and Yan Li conceived this project and supervised all experiments and data analyses. Huamin Jiang, Zhaoyi Lin and Yan Li analyzed the data and wrote the manuscript. Huamin Jiang, Zhaoyi Lin, and Jinze Li performed the experiments and analyzed the data. Ting Song, Hongyun Zang, Pengwen Li, Jiarun Li and Wenyi Hou conducted some experiments.

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