Tailoring esophageal tumor spheroids on a chip with inverse opal scaffolds for drug screening

Ruolin Shi; Xiangyi Wu; Yuanjin Zhao; Shegan Gao; Gaofeng Liang

doi:10.1088/2752-5724/ad5f47

Volume 3 Issue 3

September 2024

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Ruolin Shi, Xiangyi Wu, Yuanjin Zhao, Shegan Gao, Gaofeng Liang. Tailoring esophageal tumor spheroids on a chip with inverse opal scaffolds for drug screening[J]. Materials Futures, 2024, 3(3): 035402. doi: 10.1088/2752-5724/ad5f47

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Ruolin Shi, Xiangyi Wu, Yuanjin Zhao, Shegan Gao, Gaofeng Liang. Tailoring esophageal tumor spheroids on a chip with inverse opal scaffolds for drug screening[J]. Materials Futures, 2024, 3(3): 035402. doi: 10.1088/2752-5724/ad5f47

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Tailoring esophageal tumor spheroids on a chip with inverse opal scaffolds for drug screening

Materials Futures, Volume 3, Number 3

Received Date: 2024-02-24
Accepted Date: 2024-07-03
Rev Recd Date: 2024-06-12
Publish Date: 2024-07-17

Article information

doi: 10.1088/2752-5724/ad5f47

1.
Clinical Medical College and Henan Key Laboratory of Microbiome and Esophageal Cancer Prevention and Treatment, Henan University of Science and Technology, Luoyang 471023, People’s Republic of China
2.
Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210008, People’s Republic of China
3.
Institute of Organoids on Chips Translational Research, Henan Academy of Sciences, Zhengzhou 450003, People’s Republic of China
Data availability statement

The data that supports the findings of this study are available in the Supporting Information of this article.

Authors to whom any correspondence should be addressed.

More Information

Corresponding author: E-mail: yjzhao@seu.edu.cn; E-mail: gsg112258@163.com; E-mail: lgfeng990448@163.com

Abstract

Abstract

AbstractEsophageal cancer (EC) is characterized by high morbidity and mortality, and chemotherapy has become an indispensable means for comprehensive treatment. However, due to the limitation of the effective in vitro disease model, the development of chemotherapeutic agents still faces great challenges. In this paper, we present a novel tumor spheroid on a chip platform based on inverse opal hydrogel scaffolds to screen chemotherapeutic agents for EC treatment. With the microfluidic emulsion approach, the inverse opal hydrogel scaffolds were generated with tunable and organized pores, which could provide spatial confinement for cell growth. Thus, the suspended KYSE-70 cells could successfully form uniform cell spheroids on the inverse opal hydrogel scaffolds. It was demonstrated that the tumor cell spheroids could recapitulate 3D growth patterns in vivo and exhibited higher sensitivity to the chemotherapy agents compared with monolayer cells. Besides, by employing the scaffolds into a microfluidics to construct esophageal tumor on a chip, the device could realize high-throughput tumor cell spheroids generation and drug screening, indicating its promising role in chemotherapy drug development.
- tumor on a chip,
- inverse opal,
- microfluidics,
- chemotherapy drug screening,
- esophageal cancer

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Author contributions

G F L and Y J Z conceived and proposed the research. Y J Z designed the experimental layout. R L S analyzed all data, and conducted the experiments. R L S and X Y W wrote the manuscript, with some assistance of S G G fabricated the cell model. All authors reviewed and edited the manuscript.

Conflict of interest

The authors declare no conflict of interest.

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