Volume 3 Issue 3
September  2024
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Ruolin Shi, Xiangyi Wu, Yuanjin Zhao, Shegan Gao, Gaofeng Liang. Tailoring esophageal tumor spheroids on a chip with inverse opal scaffolds for drug screening[J]. Materials Futures, 2024, 3(3): 035402. doi: 10.1088/2752-5724/ad5f47
Citation: Ruolin Shi, Xiangyi Wu, Yuanjin Zhao, Shegan Gao, Gaofeng Liang. Tailoring esophageal tumor spheroids on a chip with inverse opal scaffolds for drug screening[J]. Materials Futures, 2024, 3(3): 035402. doi: 10.1088/2752-5724/ad5f47
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Tailoring esophageal tumor spheroids on a chip with inverse opal scaffolds for drug screening

© 2024 The Author(s). Published by IOP Publishing Ltd on behalf of the Songshan Lake Materials Laboratory
Materials Futures, Volume 3, Number 3
  • Received Date: 2024-02-24
  • Accepted Date: 2024-07-03
  • Rev Recd Date: 2024-06-12
  • Publish Date: 2024-07-17
  • AbstractEsophageal cancer (EC) is characterized by high morbidity and mortality, and chemotherapy has become an indispensable means for comprehensive treatment. However, due to the limitation of the effective in vitro disease model, the development of chemotherapeutic agents still faces great challenges. In this paper, we present a novel tumor spheroid on a chip platform based on inverse opal hydrogel scaffolds to screen chemotherapeutic agents for EC treatment. With the microfluidic emulsion approach, the inverse opal hydrogel scaffolds were generated with tunable and organized pores, which could provide spatial confinement for cell growth. Thus, the suspended KYSE-70 cells could successfully form uniform cell spheroids on the inverse opal hydrogel scaffolds. It was demonstrated that the tumor cell spheroids could recapitulate 3D growth patterns in vivo and exhibited higher sensitivity to the chemotherapy agents compared with monolayer cells. Besides, by employing the scaffolds into a microfluidics to construct esophageal tumor on a chip, the device could realize high-throughput tumor cell spheroids generation and drug screening, indicating its promising role in chemotherapy drug development.
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  • Author contributions

    G F L and Y J Z conceived and proposed the research. Y J Z designed the experimental layout. R L S analyzed all data, and conducted the experiments. R L S and X Y W wrote the manuscript, with some assistance of S G G fabricated the cell model. All authors reviewed and edited the manuscript.

    Conflict of interest

    The authors declare no conflict of interest.

  • [1]
    Shan J-Y, Che J, Song C, Zhao Y 2023 Emerging antibacterial nanozymes for wound healing Smart Med. 2 e20220025 doi: 10.1002/SMMD.20220025
    [2]
    Morgan E, Soerjomataram I, Rumgay H, Coleman H G, Thrift A P, Vignat J, Laversanne M, Ferlay J, Arnold M 2020 The global landscape of esophageal squamous cell carcinoma and esophageal adenocarcinoma incidence and mortality in 2020 and projections to 2040: new estimates from GLOBOCAN Gastroenterology 163 649-58.e2 doi: 10.1053/j.gastro.2022.05.054
    [3]
    Yang C-Y, Yu Y, Shang L, Zhao Y 2024 Flexible hemline-shaped microfibers for liquid transport Nat. Chem. Eng. 1 87-96 doi: 10.1038/s44286-023-00001-5
    [4]
    Matoska T, et al 2022 Definitive chemoradiotherapy ± induction chemotherapy in esophageal cancer: a real-world experience J. Clin. Oncol. 40 e16072 doi: 10.1200/JCO.2022.40.16_suppl.e16072
    [5]
    Zhang X-X, Lu M, Cao X, Zhao Y 2023 Functionalized microneedles for wearable electronics Smart Med. 2 e20220023 doi: 10.1002/SMMD.20220023
    [6]
    Thrumurthy S G, Chaudry M A, Thrumurthy S S D, Mughal M 2019 Oesophageal cancer: risks, prevention, and BMJ 366 l4373 doi: 10.1136/bmj.l4373
    [7]
    Huang D-Q, Cai L, Li N, Zhao Y 2023 Ultrasound-trigged micro/nanorobots for biomedical applications Smart Med. 2 e20230003 doi: 10.1002/SMMD.20230003
    [8]
    Forde P M, et al 2022 Neoadjuvant nivolumab plus chemotherapy in resectable lung cancer N. Engl. J. Med. 386 1973-85 doi: 10.1056/NEJMoa2202170
    [9]
    Ji Y, Du X, Chen M 2022 Definitive chemoradiotherapy for older patients with esophageal cancer-reply JAMA Oncol. 8 305-6 doi: 10.1001/jamaoncol.2021.6843
    [10]
    Tolabi H, Davari N, Khajehmohammadi M, Malektaj H, Nazemi K, Vahedi S, Ghalandari B, Reis R L, Ghorbani F, Oliveira J M 2023 Progress of microfluidic hydrogel-based scaffolds and organ-on-chips for the cartilage tissue engineering Adv. Mater. 35 e2208852 doi: 10.1002/adma.202208852
    [11]
    Lin X, Cai L, Cao X, Zhao Y 2023 Stimuli-responsive silk fibroin for on-demand drug delivery Smart Med. 2 e20220019 doi: 10.1002/SMMD.20220019
    [12]
    Kuzmanov U, et al 2020 Mapping signalling perturbations in myocardial fibrosis via the integrative phosphoproteomic profiling of tissue from diverse sources Nat. Biomed. 4 889-900 doi: 10.1038/s41551-020-0585-y
    [13]
    Lee S, Kim S, Koo D-J, Yu J, Cho H, Lee H, Song J M, Kim S-Y, Min D-H, Jeon N L 2021 3D microfluidic platform and tumor vascular mapping for evaluating anti-angiogenic RNAi-based nanomedicine ACS Nano 15 338-50 doi: 10.1021/acsnano.0c05110
    [14]
    Cui C, Gao H-L, Wang Z-Y, Wen S-M, Wang L-J, Fan X, Gong X, Yu S-H 2023 Controlled desiccation of preprinted hydrogel scaffolds toward complex 3D microarchitectures Adv. Mater. 35 e2207388 doi: 10.1002/adma.202207388
    [15]
    Wu X-Y, et al 2023 Chronic wounds: pathological characteristics and their stem cell-based therapies Eng. Regen. 4 81-94 doi: 10.1016/j.engreg.2022.11.004
    [16]
    Rodrigues R, Sousa P C, Gaspar J, BañobreLópez M, Lima R, Minas G 2020 Organ-on-a-chip: a preclinical microfluidic platform for the progress of nanomedicine Small 16 e2003517 doi: 10.1002/smll.202003517
    [17]
    Huang J-J, et al 2023 Emerging microfluidic technologies for sperm sorting 2023 Eng. Regen. 4 161-9 doi: 10.1016/j.engreg.2023.02.001
    [18]
    Qian Y, Zheng Y, Jin J, Wu X, Xu K, Dai M, Niu Q, Zheng H, He X, Shen J 2022 Immunoregulation in diabetic wound repair with a photoenhanced glycyrrhizic acid hydrogel scaffold Adv. Mater. 34 e2200521 doi: 10.1002/adma.202200521
    [19]
    Shao C-M, et al 2023 Organ-on-a-chip for dynamic tumor drug resistance investigation J. Chem. Eng. 460 141739 doi: 10.1016/j.cej.2023.141739
    [20]
    Zhang S Y, et al 2017 Inverse opal scaffolds and their biomedical applications Adv. Mater. 29 1701115 doi: 10.1002/adma.201701115
    [21]
    Shao C-M, Liu Y, Chi J, Wang J, Zhao Z, Zhao Y 2019 Responsive inverse opal scaffolds with biomimetic enrichment capability for cell culture Research 2019 9783793 doi: 10.34133/2019/9783793
    [22]
    Sun L-Y, Wang Y, Xu D, Zhao Y 2023 Emerging technologies for cardiac tissue engineering and artificial hearts Smart Med. 2 e20220040 doi: 10.1002/SMMD.20220040
    [23]
    Yu Y-R, et al 2021 Living materials for regenerative medicine Eng. Regen. 2 96-104 doi: 10.1016/j.engreg.2021.08.003
    [24]
    Takeshi U, et al 2024 Ionic liquid interface as a cell scaffold Adv. Mater. 36 e2310105 doi: 10.1002/adma.202310105
    [25]
    Sutterby E, Thurgood P, Baratchi S, Khoshmanesh K, Pirogova E 2020 Microfluidic skin-on-a-chip models: toward biomimetic artificial skin Small 16 e2002515 doi: 10.1002/smll.202002515
    [26]
    Chen C, Wang Y, Zhang H, Zhang H, Dong W, Sun W, Zhao Y 2021 Responsive and self-healing structural color supramolecular hydrogel patch for diabetic wound treatment Bioact. Mater. 15 194-202 doi: 10.1016/j.bioactmat.2021.11.037
    [27]
    Chen W-W, Nie M, Gan J, Xia N, Wang D, Sun L 2024 Tailoring cell sheets for biomedical applications Smart Med. 3 e20230038 doi: 10.1002/SMMD.20230038
    [28]
    Shi Z-D, et al 2022 Targeting HNRNPU to overcome cisplatin resistance in bladder cancer Mol. Cancer 21 37 doi: 10.1186/s12943-022-01517-9
    [29]
    Choi S, et al 2023 Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles Nat. Mater. 22 1039-46 doi: 10.1038/s41563-023-01611-3
    [30]
    Koenig L, et al 2022 A human stem cell-derived brain-liver chip for assessing blood-brain-barrier permeation of pharmaceutical drugs Cells 11 3295 doi: 10.3390/cells11203295
    [31]
    Yang C 2021 The prospects of tumor chemosensitivity testing at the single-cell level Drug Resist. Updates 54 100741 doi: 10.1016/j.drup.2020.100741
    [32]
    Tang Q-Q, et al 2020 Fabrication of a hydroxyapatite-PDMS microfluidic chip for bone-related cell culture and drug screening Bio. Mater. 6 169-78 doi: 10.1016/j.bioactmat.2020.07.016
    [33]
    Gokyurek M, Guler S, Gokyer S, Yazihan N, Aknc M, Gülçelik M A, Yilmaz K B, Yilgor P 2023 3D printed hydrogel scaffold promotes the formation of hormone-active engineered parathyroid tissue Biomed. Mater. 18 doi: 10.1088/1748-605X/acc99d
    [34]
    Hou Y-C, et al 2021 The therapeutic potential of MSC-EVs as a bioactive material for wound healing Eng. Regen. 2 182-94 doi: 10.1016/j.engreg.2021.11.003.
    [35]
    Zhu Y-J, Kong B, Liu R, Zhao Y 2022 Developing biomedical engineering technologies for reproductive medicine Smart Med. 1 e20220006 doi: 10.1002/SMMD.20220006
    [36]
    Dickson I, et al 2020 Multispecies liver-on-a-chip for improved drug toxicity testing Nat. Rev. Gastroenterol. Hepatol. 17 4 doi: 10.1038/s41575-019-0244-5
    [37]
    Hull S, Lou J, Lindsay C D, Navarro R S, Cai B, Brunel L G, Westerfield A D, Xia Y, Heilshorn S C 2023 3D bioprinting of dynamic hydrogel bioinks enabled by small molecule modulators Sci. Adv. 9 eade7880 doi: 10.1126/sciadv.ade7880
    [38]
    Kusumoto D, et al 2021 Anti-senescent drug screening by deep learning-based morphology senescence scoring Nat. Commun. 12 257 doi: 10.1038/s41467-020-20213-0
    [39]
    Griffin D R, et al 2021 Activating an adaptive immune response from a hydrogel scaffold imparts regenerative wound healing Nat. Mater. 20 560-9 doi: 10.1038/s41563-020-00844-w
    [40]
    Chen H-X, Guo J, Bian F, Zhao Y 2022 Microfluidic technologies for cell deformability cytometry Smart Med. 1 e20220001 doi: 10.1002/SMMD.20220001
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